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Promega pgem easy vector
Pgem Easy Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem%C2%AE-t+easy/pgem+t+easy/10__1007_slash_s13562___024___00890___4-80-7-10
Average 90 stars, based on 1 article reviews
pgem easy vector - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Clone Assay:

Article Title: Semliki Forest Virus (SFV) Self-Amplifying RNA Delivered to J774A.1 Macrophage Lineage by Its Association with a Purified Recombinant SFV Capsid Protein
Article Snippet: Mayaro virus E2 coding RNA was obtained from pGEM ® -T Easy (Promega) containing the cDNA of Mayaro Envelope 2 protein (NC_003417.1), obtained from the cloning of a custom-synthesized gBlock (IDT Technologies).

Article Title: Molecular survey of vector-borne agents in lowland tapirs (Tapirus terrestris) from Brazil reveals a new Anaplasma genotype.
Article Snippet: Although vector-borne agents have been detected in different species of wild animals, studies involving tapirs (Tapirus terrestris), the largest land mammals in Brazil, are scarce.. The aim of the present study was to investigate the occurrence and molecular identity of Anaplasmataceae agents, Coxiella burnetii and Hepatozoon spp. in blood samples of wild T. terrestris from two biomes (Cerrado and Pantanal) in Brazil.. A total of 122 blood samples from 99 tapirs were analyzed.

Article Title: Mistranslating tRNA variants have anticodon- and sex-specific impacts on Drosophila melanogaster.
Article Snippet: Second round PCR products were cloned into pGEM®-T Easy (Promega) and sequenced.

Article Title: A Rab-Kinesin12-Fused kinase module couples vesicle delivery and phragmoplast remodelling during cytokinesis in Arabidopsis
Article Snippet: For cloning of Kinesin-12F tail regions into pGADT7, Kinesin-12F tail region truncations were amplified from cDNA with primers containing XmaI and XhoI digest sites and introduced into pGEM®-T Easy (Promega).

Article Title: Development of a New Trapping System with Potential Implementation as a Tool for Mosquito-Borne Arbovirus Surveillance.
Article Snippet: To assess the card’s conservation capacity, 1 μL of pGEM®-T Easy (50 ng/μL, Promega, Madison, WI, USA) was added as a positive control before mosquito exposure.

Article Title: Suppressing the expression of glutathione S-transferase gene GSTd10 increases the sensitivity of Zeugodacus cucurbitae against β-cypermethrin.
Article Snippet: Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University, Chongqing, China Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Education Department, Guiyang University, Guiyang, China

Article Title: Analysis of chicken LGALSL (galectin-related protein) gene's proximal promoter and its control by Krüppel-like factors 3 and 7.
Article Snippet: After digestion of the amplicons with the appropriate restriction enzymes, they were either subcloned (chKLF7, chSP1) into the pGEM®-T Easy (Promega) or directly cloned into the expression vector pcDNATM3.1(+) (Thermo Fisher Scientific).

Article Title: Illuminating microbial mat assembly: Cyanobacteria and Chloroflexota cooperate to structure light-responsive biofilms
Article Snippet: A construct containing a thermostable kanamycin resistance cassette (Km Te R ), flanked by up- and down-stream regions homologous to the pilB (CYB_2143) deletion site was built in pGEM®-T easy (Promega).

Expressing:

Article Title: Semliki Forest Virus (SFV) Self-Amplifying RNA Delivered to J774A.1 Macrophage Lineage by Its Association with a Purified Recombinant SFV Capsid Protein
Article Snippet: Mayaro virus E2 coding RNA was obtained from pGEM ® -T Easy (Promega) containing the cDNA of Mayaro Envelope 2 protein (NC_003417.1), obtained from the cloning of a custom-synthesized gBlock (IDT Technologies).

Article Title: Molecular survey of vector-borne agents in lowland tapirs (Tapirus terrestris) from Brazil reveals a new Anaplasma genotype.
Article Snippet: Although vector-borne agents have been detected in different species of wild animals, studies involving tapirs (Tapirus terrestris), the largest land mammals in Brazil, are scarce.. The aim of the present study was to investigate the occurrence and molecular identity of Anaplasmataceae agents, Coxiella burnetii and Hepatozoon spp. in blood samples of wild T. terrestris from two biomes (Cerrado and Pantanal) in Brazil.. A total of 122 blood samples from 99 tapirs were analyzed.

Article Title: Mistranslating tRNA variants have anticodon- and sex-specific impacts on Drosophila melanogaster.
Article Snippet: Second round PCR products were cloned into pGEM®-T Easy (Promega) and sequenced.

Article Title: A Rab-Kinesin12-Fused kinase module couples vesicle delivery and phragmoplast remodelling during cytokinesis in Arabidopsis
Article Snippet: For cloning of Kinesin-12F tail regions into pGADT7, Kinesin-12F tail region truncations were amplified from cDNA with primers containing XmaI and XhoI digest sites and introduced into pGEM®-T Easy (Promega).

Article Title: Development of a New Trapping System with Potential Implementation as a Tool for Mosquito-Borne Arbovirus Surveillance.
Article Snippet: To assess the card’s conservation capacity, 1 μL of pGEM®-T Easy (50 ng/μL, Promega, Madison, WI, USA) was added as a positive control before mosquito exposure.

Article Title: Suppressing the expression of glutathione S-transferase gene GSTd10 increases the sensitivity of Zeugodacus cucurbitae against β-cypermethrin.
Article Snippet: Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University, Chongqing, China Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Education Department, Guiyang University, Guiyang, China

Article Title: Analysis of chicken LGALSL (galectin-related protein) gene's proximal promoter and its control by Krüppel-like factors 3 and 7.
Article Snippet: After digestion of the amplicons with the appropriate restriction enzymes, they were either subcloned (chKLF7, chSP1) into the pGEM®-T Easy (Promega) or directly cloned into the expression vector pcDNATM3.1(+) (Thermo Fisher Scientific).

Article Title: Illuminating microbial mat assembly: Cyanobacteria and Chloroflexota cooperate to structure light-responsive biofilms
Article Snippet: A construct containing a thermostable kanamycin resistance cassette (Km Te R ), flanked by up- and down-stream regions homologous to the pilB (CYB_2143) deletion site was built in pGEM®-T easy (Promega).

Plasmid Preparation:

Article Title: Semliki Forest Virus (SFV) Self-Amplifying RNA Delivered to J774A.1 Macrophage Lineage by Its Association with a Purified Recombinant SFV Capsid Protein
Article Snippet: Mayaro virus E2 coding RNA was obtained from pGEM ® -T Easy (Promega) containing the cDNA of Mayaro Envelope 2 protein (NC_003417.1), obtained from the cloning of a custom-synthesized gBlock (IDT Technologies).

Article Title: Molecular survey of vector-borne agents in lowland tapirs (Tapirus terrestris) from Brazil reveals a new Anaplasma genotype.
Article Snippet: Although vector-borne agents have been detected in different species of wild animals, studies involving tapirs (Tapirus terrestris), the largest land mammals in Brazil, are scarce.. The aim of the present study was to investigate the occurrence and molecular identity of Anaplasmataceae agents, Coxiella burnetii and Hepatozoon spp. in blood samples of wild T. terrestris from two biomes (Cerrado and Pantanal) in Brazil.. A total of 122 blood samples from 99 tapirs were analyzed.

Article Title: Mistranslating tRNA variants have anticodon- and sex-specific impacts on Drosophila melanogaster.
Article Snippet: Second round PCR products were cloned into pGEM®-T Easy (Promega) and sequenced.

Article Title: A Rab-Kinesin12-Fused kinase module couples vesicle delivery and phragmoplast remodelling during cytokinesis in Arabidopsis
Article Snippet: For cloning of Kinesin-12F tail regions into pGADT7, Kinesin-12F tail region truncations were amplified from cDNA with primers containing XmaI and XhoI digest sites and introduced into pGEM®-T Easy (Promega).

Article Title: Development of a New Trapping System with Potential Implementation as a Tool for Mosquito-Borne Arbovirus Surveillance.
Article Snippet: To assess the card’s conservation capacity, 1 μL of pGEM®-T Easy (50 ng/μL, Promega, Madison, WI, USA) was added as a positive control before mosquito exposure.

Article Title: Suppressing the expression of glutathione S-transferase gene GSTd10 increases the sensitivity of Zeugodacus cucurbitae against β-cypermethrin.
Article Snippet: Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University, Chongqing, China Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Education Department, Guiyang University, Guiyang, China

Article Title: Analysis of chicken LGALSL (galectin-related protein) gene's proximal promoter and its control by Krüppel-like factors 3 and 7.
Article Snippet: After digestion of the amplicons with the appropriate restriction enzymes, they were either subcloned (chKLF7, chSP1) into the pGEM®-T Easy (Promega) or directly cloned into the expression vector pcDNATM3.1(+) (Thermo Fisher Scientific).

Article Title: Illuminating microbial mat assembly: Cyanobacteria and Chloroflexota cooperate to structure light-responsive biofilms
Article Snippet: A construct containing a thermostable kanamycin resistance cassette (Km Te R ), flanked by up- and down-stream regions homologous to the pilB (CYB_2143) deletion site was built in pGEM®-T easy (Promega).

Cloning:

Article Title: Semliki Forest Virus (SFV) Self-Amplifying RNA Delivered to J774A.1 Macrophage Lineage by Its Association with a Purified Recombinant SFV Capsid Protein
Article Snippet: Mayaro virus E2 coding RNA was obtained from pGEM ® -T Easy (Promega) containing the cDNA of Mayaro Envelope 2 protein (NC_003417.1), obtained from the cloning of a custom-synthesized gBlock (IDT Technologies).

Article Title: Molecular survey of vector-borne agents in lowland tapirs (Tapirus terrestris) from Brazil reveals a new Anaplasma genotype.
Article Snippet: Although vector-borne agents have been detected in different species of wild animals, studies involving tapirs (Tapirus terrestris), the largest land mammals in Brazil, are scarce.. The aim of the present study was to investigate the occurrence and molecular identity of Anaplasmataceae agents, Coxiella burnetii and Hepatozoon spp. in blood samples of wild T. terrestris from two biomes (Cerrado and Pantanal) in Brazil.. A total of 122 blood samples from 99 tapirs were analyzed.

Article Title: Mistranslating tRNA variants have anticodon- and sex-specific impacts on Drosophila melanogaster.
Article Snippet: Second round PCR products were cloned into pGEM®-T Easy (Promega) and sequenced.

Article Title: A Rab-Kinesin12-Fused kinase module couples vesicle delivery and phragmoplast remodelling during cytokinesis in Arabidopsis
Article Snippet: For cloning of Kinesin-12F tail regions into pGADT7, Kinesin-12F tail region truncations were amplified from cDNA with primers containing XmaI and XhoI digest sites and introduced into pGEM®-T Easy (Promega).

Article Title: Development of a New Trapping System with Potential Implementation as a Tool for Mosquito-Borne Arbovirus Surveillance.
Article Snippet: To assess the card’s conservation capacity, 1 μL of pGEM®-T Easy (50 ng/μL, Promega, Madison, WI, USA) was added as a positive control before mosquito exposure.

Article Title: Suppressing the expression of glutathione S-transferase gene GSTd10 increases the sensitivity of Zeugodacus cucurbitae against β-cypermethrin.
Article Snippet: Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University, Chongqing, China Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Education Department, Guiyang University, Guiyang, China

Article Title: Analysis of chicken LGALSL (galectin-related protein) gene's proximal promoter and its control by Krüppel-like factors 3 and 7.
Article Snippet: After digestion of the amplicons with the appropriate restriction enzymes, they were either subcloned (chKLF7, chSP1) into the pGEM®-T Easy (Promega) or directly cloned into the expression vector pcDNATM3.1(+) (Thermo Fisher Scientific).

Article Title: Illuminating microbial mat assembly: Cyanobacteria and Chloroflexota cooperate to structure light-responsive biofilms
Article Snippet: A construct containing a thermostable kanamycin resistance cassette (Km Te R ), flanked by up- and down-stream regions homologous to the pilB (CYB_2143) deletion site was built in pGEM®-T easy (Promega).

Amplification:

Article Title: Semliki Forest Virus (SFV) Self-Amplifying RNA Delivered to J774A.1 Macrophage Lineage by Its Association with a Purified Recombinant SFV Capsid Protein
Article Snippet: Mayaro virus E2 coding RNA was obtained from pGEM ® -T Easy (Promega) containing the cDNA of Mayaro Envelope 2 protein (NC_003417.1), obtained from the cloning of a custom-synthesized gBlock (IDT Technologies).

Article Title: Molecular survey of vector-borne agents in lowland tapirs (Tapirus terrestris) from Brazil reveals a new Anaplasma genotype.
Article Snippet: Although vector-borne agents have been detected in different species of wild animals, studies involving tapirs (Tapirus terrestris), the largest land mammals in Brazil, are scarce.. The aim of the present study was to investigate the occurrence and molecular identity of Anaplasmataceae agents, Coxiella burnetii and Hepatozoon spp. in blood samples of wild T. terrestris from two biomes (Cerrado and Pantanal) in Brazil.. A total of 122 blood samples from 99 tapirs were analyzed.

Article Title: Mistranslating tRNA variants have anticodon- and sex-specific impacts on Drosophila melanogaster.
Article Snippet: Second round PCR products were cloned into pGEM®-T Easy (Promega) and sequenced.

Article Title: A Rab-Kinesin12-Fused kinase module couples vesicle delivery and phragmoplast remodelling during cytokinesis in Arabidopsis
Article Snippet: For cloning of Kinesin-12F tail regions into pGADT7, Kinesin-12F tail region truncations were amplified from cDNA with primers containing XmaI and XhoI digest sites and introduced into pGEM®-T Easy (Promega).

Article Title: Development of a New Trapping System with Potential Implementation as a Tool for Mosquito-Borne Arbovirus Surveillance.
Article Snippet: To assess the card’s conservation capacity, 1 μL of pGEM®-T Easy (50 ng/μL, Promega, Madison, WI, USA) was added as a positive control before mosquito exposure.

Article Title: Suppressing the expression of glutathione S-transferase gene GSTd10 increases the sensitivity of Zeugodacus cucurbitae against β-cypermethrin.
Article Snippet: Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University, Chongqing, China Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Education Department, Guiyang University, Guiyang, China

Article Title: Analysis of chicken LGALSL (galectin-related protein) gene's proximal promoter and its control by Krüppel-like factors 3 and 7.
Article Snippet: After digestion of the amplicons with the appropriate restriction enzymes, they were either subcloned (chKLF7, chSP1) into the pGEM®-T Easy (Promega) or directly cloned into the expression vector pcDNATM3.1(+) (Thermo Fisher Scientific).

Article Title: Illuminating microbial mat assembly: Cyanobacteria and Chloroflexota cooperate to structure light-responsive biofilms
Article Snippet: A construct containing a thermostable kanamycin resistance cassette (Km Te R ), flanked by up- and down-stream regions homologous to the pilB (CYB_2143) deletion site was built in pGEM®-T easy (Promega).

Polymerase Chain Reaction:

Article Title: Semliki Forest Virus (SFV) Self-Amplifying RNA Delivered to J774A.1 Macrophage Lineage by Its Association with a Purified Recombinant SFV Capsid Protein
Article Snippet: Mayaro virus E2 coding RNA was obtained from pGEM ® -T Easy (Promega) containing the cDNA of Mayaro Envelope 2 protein (NC_003417.1), obtained from the cloning of a custom-synthesized gBlock (IDT Technologies).

Article Title: Molecular survey of vector-borne agents in lowland tapirs (Tapirus terrestris) from Brazil reveals a new Anaplasma genotype.
Article Snippet: Although vector-borne agents have been detected in different species of wild animals, studies involving tapirs (Tapirus terrestris), the largest land mammals in Brazil, are scarce.. The aim of the present study was to investigate the occurrence and molecular identity of Anaplasmataceae agents, Coxiella burnetii and Hepatozoon spp. in blood samples of wild T. terrestris from two biomes (Cerrado and Pantanal) in Brazil.. A total of 122 blood samples from 99 tapirs were analyzed.

Article Title: Mistranslating tRNA variants have anticodon- and sex-specific impacts on Drosophila melanogaster.
Article Snippet: Second round PCR products were cloned into pGEM®-T Easy (Promega) and sequenced.

Article Title: A Rab-Kinesin12-Fused kinase module couples vesicle delivery and phragmoplast remodelling during cytokinesis in Arabidopsis
Article Snippet: For cloning of Kinesin-12F tail regions into pGADT7, Kinesin-12F tail region truncations were amplified from cDNA with primers containing XmaI and XhoI digest sites and introduced into pGEM®-T Easy (Promega).

Article Title: Development of a New Trapping System with Potential Implementation as a Tool for Mosquito-Borne Arbovirus Surveillance.
Article Snippet: To assess the card’s conservation capacity, 1 μL of pGEM®-T Easy (50 ng/μL, Promega, Madison, WI, USA) was added as a positive control before mosquito exposure.

Article Title: Suppressing the expression of glutathione S-transferase gene GSTd10 increases the sensitivity of Zeugodacus cucurbitae against β-cypermethrin.
Article Snippet: Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University, Chongqing, China Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Education Department, Guiyang University, Guiyang, China

Article Title: Analysis of chicken LGALSL (galectin-related protein) gene's proximal promoter and its control by Krüppel-like factors 3 and 7.
Article Snippet: After digestion of the amplicons with the appropriate restriction enzymes, they were either subcloned (chKLF7, chSP1) into the pGEM®-T Easy (Promega) or directly cloned into the expression vector pcDNATM3.1(+) (Thermo Fisher Scientific).

Article Title: Illuminating microbial mat assembly: Cyanobacteria and Chloroflexota cooperate to structure light-responsive biofilms
Article Snippet: A construct containing a thermostable kanamycin resistance cassette (Km Te R ), flanked by up- and down-stream regions homologous to the pilB (CYB_2143) deletion site was built in pGEM®-T easy (Promega).

Virus:

Article Title: Semliki Forest Virus (SFV) Self-Amplifying RNA Delivered to J774A.1 Macrophage Lineage by Its Association with a Purified Recombinant SFV Capsid Protein
Article Snippet: Mayaro virus E2 coding RNA was obtained from pGEM ® -T Easy (Promega) containing the cDNA of Mayaro Envelope 2 protein (NC_003417.1), obtained from the cloning of a custom-synthesized gBlock (IDT Technologies).

Article Title: Molecular survey of vector-borne agents in lowland tapirs (Tapirus terrestris) from Brazil reveals a new Anaplasma genotype.
Article Snippet: Although vector-borne agents have been detected in different species of wild animals, studies involving tapirs (Tapirus terrestris), the largest land mammals in Brazil, are scarce.. The aim of the present study was to investigate the occurrence and molecular identity of Anaplasmataceae agents, Coxiella burnetii and Hepatozoon spp. in blood samples of wild T. terrestris from two biomes (Cerrado and Pantanal) in Brazil.. A total of 122 blood samples from 99 tapirs were analyzed.

Article Title: Mistranslating tRNA variants have anticodon- and sex-specific impacts on Drosophila melanogaster.
Article Snippet: Second round PCR products were cloned into pGEM®-T Easy (Promega) and sequenced.

Article Title: A Rab-Kinesin12-Fused kinase module couples vesicle delivery and phragmoplast remodelling during cytokinesis in Arabidopsis
Article Snippet: For cloning of Kinesin-12F tail regions into pGADT7, Kinesin-12F tail region truncations were amplified from cDNA with primers containing XmaI and XhoI digest sites and introduced into pGEM®-T Easy (Promega).

Article Title: Development of a New Trapping System with Potential Implementation as a Tool for Mosquito-Borne Arbovirus Surveillance.
Article Snippet: To assess the card’s conservation capacity, 1 μL of pGEM®-T Easy (50 ng/μL, Promega, Madison, WI, USA) was added as a positive control before mosquito exposure.

Article Title: Suppressing the expression of glutathione S-transferase gene GSTd10 increases the sensitivity of Zeugodacus cucurbitae against β-cypermethrin.
Article Snippet: Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University, Chongqing, China Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Education Department, Guiyang University, Guiyang, China

Article Title: Analysis of chicken LGALSL (galectin-related protein) gene's proximal promoter and its control by Krüppel-like factors 3 and 7.
Article Snippet: After digestion of the amplicons with the appropriate restriction enzymes, they were either subcloned (chKLF7, chSP1) into the pGEM®-T Easy (Promega) or directly cloned into the expression vector pcDNATM3.1(+) (Thermo Fisher Scientific).

Article Title: Illuminating microbial mat assembly: Cyanobacteria and Chloroflexota cooperate to structure light-responsive biofilms
Article Snippet: A construct containing a thermostable kanamycin resistance cassette (Km Te R ), flanked by up- and down-stream regions homologous to the pilB (CYB_2143) deletion site was built in pGEM®-T easy (Promega).



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